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Observation of a higher JAK2 V617F homozygous mutated clone in polycythemia vera compared to essential thrombocythemia

LIU Hongxing, TONG Chunrong, CAI Peng, GU Jiangying, LIN Yuehui, TENG Wen, WANG He, ZHANG Ying, ZHU Ping

《医学前沿（英文）》 2008年 第2卷 第3期   页码 309-313 doi: 10.1007/s11684-008-0059-1

摘要： Single-tube bi-directional allele specific amplification (SB-ASA) and real-time quantitative polymerase chain reaction (RQ-PCR) assays were developed and performed for JAK2V617F detection on 40 polycythemia vera (PV) samples, 31 essential thrombocythemia (ET) samples, 40 acute leukemia samples, and 40 healthy control samples. Differences between detect limitations of the two assays and their influence on the mutation detection rate were analyzed. The results showed that in some samples, the JAK2V617F burden was as low as nearly 1%, and thus more JAK2V617F-positive samples were detected by RQ-PCR than by SB-ASA assay due to the former higher detect limitation. Mutation allele ratios in PV and ET samples and their relevance to biological characteristics were also analyzed. The results showed that the mutation allele ratio was 0.436 ± 0.261 in PV, higher than the 0.216 ± 0.207 in ET; percentage of certainly homozygous mutation carriers in PV was 40.54%, higher than the 10% in ET. However, statistical analysis showed no relevance between mutation allele burden and sex or age. Our result shows that the pathogenesis of PV and ET may be related to the mutation allele burden of JAK2V617F.

关键词： specific amplification     essential thrombocythemia     Single-tube bi-directional     JAK2V617F-positive     JAK2V617F detection    

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海洋渔业碳汇及其扩增战略

唐启升,刘 慧

《中国工程科学》 2016年 第18卷 第3期   页码 68-73 doi: 10.15302/J-SSCAE-2016.03.011

摘要：

本文介绍了海洋渔业碳汇和碳汇渔业的定义、研究现状、问题及技术需求。与林业碳汇比较结果表明，海洋渔业碳汇不仅有高效的特性，还有扩增的潜质。为此，提出海洋渔业碳汇扩增对策建议，主要包括：查明我国海洋渔业碳汇潜力及动态机制；大力发展以海水养殖为主体的碳汇渔业；加强近海自然碳汇及其环境的养护和管理；实施相应的渔业碳汇扩增工程建设，促进环境友好型水产养殖业发展。

关键词： 海洋渔业碳汇     碳汇渔业     碳汇扩增     养护和管理     贝藻养殖    

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Influence of sprinkler irrigation droplet diameter, application intensity and specific power on flower

Yisheng ZHANG, Delan ZHU

《农业科学与工程前沿（英文）》 2017年 第4卷 第2期   页码 165-171 doi: 10.15302/J-FASE-2017145

摘要： To determine the main parameters of droplet strike damage and avoid flower injury due to the unsuitable practices during sprinkler irrigation, an indoor experiment of irrigation droplet impact on cyclamen was conducted. The influences of different parameters such as droplet diameter, application intensity, specific power on flower strike damage was analyzed using Image Pro-Plus software to compute strike damage area and define damage level by sense-analysis. The results showed that a damage area of <1% represents a safe irrigation level, 1%–3% slight damage level, 3%–6% moderate damage level, and>6% heavy damage level. Equations of application intensity, specific power with sprinkler irrigation time and flower injury ratio were regressed against parameters which cause impact damages. The results indicated that specific power has a significant correlation with injury, and flower damage area increased as the increasing of the value of specific power for the same irrigation time. Application intensity was also correlated with injury when the droplet diameter was larger than 1 mm. When the duration of sprinkler irrigation was 1, 5 and 10 min, the threshold of impinging damage of application intensity was 25.30, 5.01 and 1.64 mm·h and the specific power was 0.467×10 , 9.340×10 and 3.110×10 W·m . These results provide a reference for determining the suitable values of sprinkler properties in operation design.

关键词： application intensity     damage     floriculture     flowers     specific power     sprinkler irrigation    

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The combined effects of biomass and temperature on maximum specific ammonia oxidation rate in domestic

《环境科学与工程前沿（英文）》 2021年 第15卷 第6期 doi: 10.1007/s11783-021-1411-9

摘要：

• Actual SAORs was determined using MLVSS and temperature.

关键词： Specific ammonia oxidation rate     Sequencing batch reactor     Biomass     Temperature coefficient     Model simulation    

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比能法在渤海海峡隧道TBM 施工中的应用分析

高攀,邹翀

《中国工程科学》 2013年 第15卷 第12期   页码 73-79

摘要：

拟建的渤海海峡隧道采用隧道掘进机（TBM）施工极具挑战性，其中关键之一就是TBM的掘进速率预测问题。本文将渤海海峡隧道和澳大利亚Clem Jones 隧道进行工程类比，并介绍了比能法在Clem Jones 隧道的应用情况。在Clem Jones 隧道施工过程中，相关研究人员运用比能法对TBM的可掘性进行了分析及预测，从后期的掘进情况来看比能法的预测结果与实际施工吻合得较好。本文在Clem Jones 隧道和渤海海峡隧道对比分析的基础上，认为运用比能法预测TBM的可掘性同样适用于渤海海峡隧道的TBM施工。

关键词： TBM     比能     凿岩速率指数     掘进速率    

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An integrated approach to site-specific management zone delineation

Yuxin MIAO, David J. MULLA, Pierre C. ROBERT

《农业科学与工程前沿（英文）》 2018年 第5卷 第4期   页码 432-441 doi: 10.15302/J-FASE-2018230

摘要：

Dividing fields into a few relatively homogeneous management zones (MZ) is a practical and cost-effective approach to precision agriculture. There are three basic approaches to MZ delineation using soil and/or landscape properties, yield information, and both sources of information. The objective of this study is to propose an integrated approach to delineating site-specific MZ using relative elevation, organic matter, slope, electrical conductivity, yield spatial trend map, and yield temporal stability map (ROSE-YSTTS) and evaluate it against two other approaches using only soil and landscape information (ROSE) or clustering multiple year yield maps (CMYYM). The study was carried out on two no-till corn-soybean rotation fields in eastern Illinois, USA. Two years of nitrogen (N) rate experiments were conducted in Field B to evaluate the delineated MZs for site-specific N management. It was found that in general the ROSE approach was least effective in accounting for crop yield variability (8.0%–9.8%), while the CMYYM approach was least effective in accounting for soil and landscape (8.9%–38.1%), and soil nutrient and pH variability (9.4%–14.5%). The integrated ROSE-YSTTS approach was reasonably effective in accounting for the three sources of variability (38.6%–48.9%, 16.1%–17.3% and 13.2%–18.7% for soil and landscape, nutrient and pH, and yield variability, respectively), being either the best or second best approach. It was also found that the ROSE-YSTTS approach was effective in defining zones with high, medium and low economically optimum N rates. It is concluded that the integrated ROSE-YSTTS approach combining soil, landscape and yield spatial-temporal variability information can overcome the weaknesses of approaches using only soil, landscape or yield information, and is more robust for MZ delineation. It also has the potential for site-specific N management for improved economic returns. More studies are needed to further evaluate their appropriateness for precision N and crop management.

关键词： economically optimum nitrogen rate     fuzzy cluster analysis     precision nitrogen management     site-specific management     soil landscape property     yield map    

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Water-dispersible nano-pollutions reshape microbial metabolism in type-specific manners: A metabolic

《环境科学与工程前沿（英文）》 2022年 第16卷 第9期 doi: 10.1007/s11783-022-1548-1

摘要：

• Water-dispersible nano-pollutions exhibit type-specific toxic effects on E. coli.

关键词： Nano-toxicity     Nano-plastics     Quantum dots     Microbial metabolite     Metabolic dysregulation    

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River restoration challenges with a specific view on hydromorphology

Jianhua LI, Stephan HOERBINGER, Clemens WEISSTEINER, Lingmin PENG, Hans Peter RAUCH

《结构与土木工程前沿（英文）》 2020年 第14卷 第5期   页码 1033-1038 doi: 10.1007/s11709-020-0665-9

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Application of StrucGP in medical immunology: site-specific -glycoproteomic analysis of macrophages

《医学前沿（英文）》 2023年 第17卷 第2期   页码 304-316 doi: 10.1007/s11684-022-0964-8

摘要： The structure of N-glycans on specific proteins can regulate innate and adaptive immunity via sensing environmental signals. Meanwhile, the structural diversity of N-glycans poses analytical challenges that limit the exploration of specific glycosylation functions. In this work, we used THP-1-derived macrophages as examples to show the vast potential of a N-glycan structural interpretation tool StrucGP in N-glycoproteomic analysis. The intact glycopeptides of macrophages were enriched and analyzed using mass spectrometry (MS)-based glycoproteomic approaches, followed by the large-scale mapping of site-specific glycan structures via StrucGP. Results revealed that bisected GlcNAc, core fucosylated, and sialylated glycans (e.g., HexNAc4Hex5Fuc1Neu5Ac1, N4H5F1S1) were increased in M1 and M2 macrophages, especially in the latter. The findings indicated that these structures may be closely related to macrophage polarization. In addition, a high level of glycosylated PD-L1 was observed in M1 macrophages, and the LacNAc moiety was detected at Asn-192 and Asn-200 of PD-L1, and Asn-200 contained Lewis epitopes. The precision structural interpretation of site-specific glycans and subsequent intervention of target glycoproteins and related glycosyltransferases are of great value for the development of new diagnostic and therapeutic approaches for different diseases.

关键词： macrophage     glycoproteome     glycopeptides     N-glycan structures     PD-L1    

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Using RAPD in genotyping and transmission detection

ZHANG Tiejun, ZHOU Xiaoming, ZHANG Tao, YU Shunzhang, JIANG Qingwu, CHEN Yue

《医学前沿（英文）》 2008年 第2卷 第3期   页码 269-275 doi: 10.1007/s11684-008-0051-9

摘要： The aim of this paper is to develop an applicable Random Amplified Polymorphic DNAs (RAPD) method for genotyping strain, and discuss the possibility of using the RAPD method to trace strain transmission route. Four different pretreatment methods were used on the genomic DNA component, and the best adaptive extract method was selected for RAPD. Different RAPD primers sequence was used for amplification and their differentiating capabilities for strains were compared. Applicable RAPD primer was selected for genotyping and then applied into transmission detection. The results show that the so called cetyltrimethylammonium bromide (CTAB) method for extracting genomic DNA could give integrated genomic DNA and give out relatively better RAPD fingerprint maps, subsequently, using selected RAPD primer could give out a group of amplification polymerase chain reaction bands. The fingerprint maps from different strains were distinctive. Some main segments were common to all the strains tested. Some segments were different among the strains. According to the fingerprint maps and similarity index of different isolates, isolates from a pair of sex-partners were very similar. Based on these findings, the best extracting method and suitable RAPD primer were chosen. The RAPD fingerprint maps could type effectively and could be used as an additional approach in molecular epidemiology for tracing infection sources.

关键词： amplification polymerase     CTAB     sequence     cetyltrimethylammonium     RAPD    

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Construction of 6HRE-GFAP-Baxα system specific for glioma gene therapy

TIAN Yongji, LI Guilin, GAO Jun, WANG Renzhi, KONG Yanguo, ZHANG Zhenxing, LI Shifang, TIAN Shiqiang, DOU Wanchen, ZHANG Bo

《医学前沿（英文）》 2007年 第1卷 第1期   页码 49-53 doi: 10.1007/s11684-007-0010-x

摘要： The aim of this paper is to construct a specific and high-performance gene therapy system for glioma. We constructed a combined promoter 6HRE-GFAP (Hypoxia Responsive Element, Glial Fibrillary Acidic Protein) by gene recombination techniques according to the hypoxia microenvironment in glioma and tested its efficacy and specificity in cultured cells, and then constructed GFAP-Baxα gene expressing system and determined its promoting of apoptosis in glioma cells. Our primary results showed that in U251 and BT325 cell lines, the activity of GFAP promoter was 16.40 and 4.73-fold of the promoter of hTERT (Human Telomerase Reverse Transcriptase), respectively. The activities of 6HRE-GFAP-promoter increased by 3.08 and 1.30-fold under 2% O condition compared with those under 18% O condition, while under 0.2% O condition increased by 8.90 and 2.69-fold, respectively. The glioma cells showed typical apoptotic signs 90 hours after the transient transfection of GFAP-Baxα. In these primary experiments, it showed that 6HRE-GFAPBaxα system could promote glioma cell apoptosis. It was specific and effective for glioma gene therapy.

关键词： GFAP-Baxα     BT325     Fibrillary     apoptotic     specificity    

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Construction of Hsp90β gene specific silencing plasmid and its transfection efficiency

JI Yewei, NIE Bin, LI Ping, ZHOU Yuanguo, XU Xiaoyu

《医学前沿（英文）》 2007年 第1卷 第3期   页码 253-257 doi: 10.1007/s11684-007-0048-9

摘要： The purpose of this work was to construct the plasmid that could direct the synthesis of siRNA-like transcripts and thus mediate strong and specific repression of human heat shock protein 90β (Hsp90β) gene expression and to compare the transfection efficiency of the plasmids in varying conditions of transfection. Three 64 nt oligos corresponding to different regions of the target gene were chemically synthesized and annealed and were then ligated with pSUPER EGFP1 plasmid and double-digested with HindIII and Π. Recombinant plasmids were transformed into , DH5a, and the colonies were picked and grown in the Amp-agarose. The presence of positive clones was checked by the means of endodigestion and sequencing. Three cell strains, HepG2, Human umbilicus vein endothelium cells (HUVEC) and HeK293, were cultured. Then the plasmids were transfected into the cells at different ratios of plasmid to Lipofectamine. The transfection efficiency was measured by detection of enhanced green fluorescence protein (EGFP). The presence of positive recombinant clones were verified by double-digestion and sequencing. The bases inserted into the plasmids were correct and the positive colonies were named pSuper-Hsp90β1, pSuper-Hsp90β2 and pSuper-Hsp90β3. After optimizing the ratio of plasmid to Lipofectamine, we achieved high transfection efficiency in HeK293 cells. Transfection efficiency was still low in the HepG2 cells. In conclusion, the si-RNA-synthesizing plasmids targeting Hsp90β were constructed and transfected into cells with different transfection efficiency.

关键词： HindIII     transfection efficiency     detection     enhanced     different    

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Modeling of specific structure crystallization coupling with dissolution

Yuanhui JI, Hongliang QIAN, Chang LIU, Xiaohua LU, Xin FENG, Xiaoyan JI,

《化学科学与工程前沿（英文）》 2010年 第4卷 第1期   页码 52-56 doi: 10.1007/s11705-009-0301-7

摘要： In this paper, the research framework for specific structure crystallization modeling has been proposed in which four steps are required in order to investigate the rigorous crystallization modeling by thermodynamics. The first is the activity coefficient model of the solution, the second is Solid-Liquid equilibrium, the third and fourth are the dissolution and crystallization kinetics modeling, respectively. Our investigations show that the mechanisms of complex structure formation and microphase transition can be analyzed by combining the dissolution and crystallization kinetics modeling. Moreover, the formation mechanism of the porous KCl has been analyzed, which may provide a reference for the porous structure formation in the advanced material synthesis.

关键词： structure crystallization     activity coefficient     reference     KCl     dissolution    

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智能手机成像的晶片上基于逆转录环介导等温扩增(RT-LAMP)技术的全血中HIV-1检测 Article

Gregory L. Damhorst,Carlos Duarte-Guevara,Weili Chen,Tanmay Ghonge,Brian T. Cunningham,Rashid Bashir

《工程（英文）》 2015年 第1卷 第3期   页码 324-335 doi: 10.15302/J-ENG-2015072

摘要：

病毒载量测量对于人类免疫缺陷病毒 (HIV) 阳性患者长期临床护理来说是一个必不可少的工具。然而，考虑到病毒载量测量所需的仪器体积、成本和操作的复杂性，在医疗基础设施较差的偏远地区 (尤其是在被HIV感染人群比例较高的地区) 普及标准的病毒载量测量仪器是较为困难的。为提高该检测方法的普及性，人们已经开始开发可以进行即时检测的病毒载量检测平台，然而尚没有解决办法能够同时满足低成本、便携、易于操作等多种实际要求。本文通过运用微流体和微型硅晶片平台，对经过最低程度处理的含有HIV的全血样本进行了逆转录环介导等温扩增 (RT-LAMP)，并利用智能手机进行了荧光检测。集成实验检测结果表明，一滴约60 nL的反应液滴中仅有的3个病毒依然可以通过RT-LAMP技术被检测到，这相当于每微升全血样品中只有670个病毒。该技术在数字化RT-LAMP方法上具有重要意义，扩展该技术能够实现对HIV阳性患者在临床护理中采集指血进行病毒载量检测。研究结果显示，病毒载量检测过程所需的各个步骤，从血滴的准备到RT-LAMP反应的成像，都可以集成为晶片实验并且可以和移动设备兼容。

关键词： 人类免疫缺陷病毒(HIV)     病毒载量     环介导等温扩增     智能手机     即时检测    

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Cytochrome P450 monooxygenase specific activity reduction in wheat

Kangxin HE,Qixing ZHOU

《环境科学与工程前沿（英文）》 2016年 第10卷 第2期   页码 270-275 doi: 10.1007/s11783-015-0813-y

摘要： Roxithromycin, as widely used medicine and livestock growth promoter, arouses concern because its occurrence and persistence in soil environments. However, effects of roxithromycin in higher plants are still vague. Accordingly, we hypothesized that roxithromycin-contaminated soil may exhibits ecotoxicological effects in wheat ( ). In this study, effects induced by a gradient concentration of roxithromycin stress (0.01, 0.1, 1, 10, and 100 mg·kg ) was investigated in a 7-d soil test in . Results indicated that the specific activity of cytochrome P450 (CYP450) monooxygenase was decreased dramatically with the concentration of roxithromycin in soil. The IC value was 8.78 mg·kg of roxithromycin. On the contrary, the growth related endpoints (i.e., the germination percentage, the biomass and the height), the content related endpoints (i.e., soluble protein content and CYP450 content), and the superoxide dismutase (SOD) activity failed to reveal the roxithromycin-induced effects. Further analysis revealed that the CYP450 monooxygenase specific activity reduction was enzymatic mechanism mediated, other than oxidative stress induced. We conclude that the soil roxithromycin declined the CYP450 monooxygenase activity in by the inhibition of the enzymatic mechanism. Further efforts can include, but are not limited to, investigation of joint effects induced by combined exposure of roxithromycin and the pesticides and evaluation of the similar effects in other higher plants.

关键词： roxithromycin     toxic effect     cytochrome P450 monooxygenase     soil environment     Triticum aestivum     biomarker    

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标题 作者 时间 类型 操作

Observation of a higher JAK2 V617F homozygous mutated clone in polycythemia vera compared to essential thrombocythemia

LIU Hongxing, TONG Chunrong, CAI Peng, GU Jiangying, LIN Yuehui, TENG Wen, WANG He, ZHANG Ying, ZHU Ping

期刊论文

海洋渔业碳汇及其扩增战略

唐启升,刘 慧

期刊论文

Influence of sprinkler irrigation droplet diameter, application intensity and specific power on flower

Yisheng ZHANG, Delan ZHU

期刊论文

The combined effects of biomass and temperature on maximum specific ammonia oxidation rate in domestic

期刊论文

比能法在渤海海峡隧道TBM 施工中的应用分析

高攀,邹翀

期刊论文

An integrated approach to site-specific management zone delineation

Yuxin MIAO, David J. MULLA, Pierre C. ROBERT

期刊论文

Water-dispersible nano-pollutions reshape microbial metabolism in type-specific manners: A metabolic

期刊论文

River restoration challenges with a specific view on hydromorphology

Jianhua LI, Stephan HOERBINGER, Clemens WEISSTEINER, Lingmin PENG, Hans Peter RAUCH

期刊论文

Application of StrucGP in medical immunology: site-specific -glycoproteomic analysis of macrophages

期刊论文

Using RAPD in genotyping and transmission detection

ZHANG Tiejun, ZHOU Xiaoming, ZHANG Tao, YU Shunzhang, JIANG Qingwu, CHEN Yue

期刊论文

Construction of 6HRE-GFAP-Baxα system specific for glioma gene therapy

TIAN Yongji, LI Guilin, GAO Jun, WANG Renzhi, KONG Yanguo, ZHANG Zhenxing, LI Shifang, TIAN Shiqiang, DOU Wanchen, ZHANG Bo

期刊论文

Construction of Hsp90β gene specific silencing plasmid and its transfection efficiency

JI Yewei, NIE Bin, LI Ping, ZHOU Yuanguo, XU Xiaoyu

期刊论文

Modeling of specific structure crystallization coupling with dissolution

Yuanhui JI, Hongliang QIAN, Chang LIU, Xiaohua LU, Xin FENG, Xiaoyan JI,

期刊论文

智能手机成像的晶片上基于逆转录环介导等温扩增(RT-LAMP)技术的全血中HIV-1检测

Gregory L. Damhorst,Carlos Duarte-Guevara,Weili Chen,Tanmay Ghonge,Brian T. Cunningham,Rashid Bashir

期刊论文

Cytochrome P450 monooxygenase specific activity reduction in wheat

Kangxin HE,Qixing ZHOU

期刊论文